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美國NIGHTSEA專門致力于研究和開發(fā)熒光觀測裝備。NIGHTSEA轉基因生物觀測利器包括：SFA-RB體視顯微鏡熒光適配器，DFP-1雙熒光蛋白手電筒系列，BLS2單熒光蛋白手電筒系列等。
（一）美國NIGHTSEA轉基因生物觀測利器的應用之——轉基因生物觀測：如小鼠幼崽，果蠅幼蟲，線蟲，斑馬魚，等等。
1. 美國NIGHTSEA轉基因生物觀測利器—觀測轉基因果蠅
2. 美國NIGHTSEA轉基因生物觀測利器—觀測轉基因斑馬魚
3. 美國NIGHTSEA轉基因生物觀測利器—觀測轉基因爪蟾
4. 美國NIGHTSEA轉基因生物觀測利器—觀測轉基因線蟲
5.美國NIGHTSEA轉基因生物觀測利器—觀測轉基因蠑螈
6.美國NIGHTSEA轉基因生物觀測利器—觀測轉基因擬南芥種子
（二）美國NIGHTSEA轉基因生物觀測利器的應用之——樣本預篩選和輔助解剖
熒光是細胞生物學、神經(jīng)生物學等多個研究領域中廣泛使用的工具。nightsea的DFP-1, BLS2和SFA-RB可以用于樣本的預篩選、輔助解剖等，下面列舉幾個實例：
1. 熒光蛋白標記的工程病毒載體在神經(jīng)元功能研究中應用十分廣泛，常通過顱內(nèi)注射研究特定腦區(qū)的功能。在此中，常需要對病毒表達區(qū)域進行快速解剖用于特定的生化分析。為了提高快速解剖定位度，研究人員選用nightsea的BLS2 Bluestar手電筒觀測系列，通過配合nightsea濾光眼鏡準確定位注射表達了熒光蛋白的伏核區(qū)(文獻– Xuan Li and Marina E. Wolf, 2011. Visualization of virus-infected brain regions using a GFP-illuminating flashlight enables accurate and rapid dissection for biochemical analysis. J. of Neuroscience Methods, Vol. 201, Issue 1, pp. 177-179. )
EGFP標記的慢病毒載體注射進小鼠大腦區(qū)域（Marina Wolf, Rosalind Franklin University）
2. 研究者從小鼠大腦中提取GFP標記的背紋體。這項困難的工作因有了NIGHTSEA的DFP-1或BLS2（手電筒和眼鏡組合）而變得簡單：很容易看到大腦中的目標區(qū)域，讓解剖更準確、。
小鼠大腦中GFP標記的背紋體（Charles Mazel. Sample photographed at laboratory of Stefano Vicini, Georgetown University）
3. 用DFP-1雙熒光蛋白手電筒，BLS2單熒光蛋白手電筒，或SFA-RB體視顯微鏡熒光適配器，可以快速檢測樣本是否成功染色（Alexa Fluor 488 Phalloidin標記），如下圖：
兔腰肌的肌纖維（用Alexa Fluor 488 Phalloidin標記）
左圖：白光觀測 右圖：Royal Blue熒光觀測（Motic SMZ-186體視顯微鏡觀測）
4.用SFA-RB體視顯微鏡熒光適配器以斑馬魚為模型，觀測不同有害化學物（醫(yī)藥、農(nóng)藥、食品添加劑等）對大腦發(fā)育的影響。觀測圖片如下：
基因斑馬魚大腦共聚焦圖像
圖片由Robert Thorn（Creton Lab, 布朗大學）提供
5. 熒光是珊瑚采集研究的重要工具。現(xiàn)在，可以用經(jīng)濟又實惠的SFA-RB體視顯微鏡熒光適配器安裝到體視顯微鏡上檢測珊瑚。
下面的圖片是通過立體顯微鏡觀看珊瑚蟲，由UNCW威爾明頓納大學Alina Szmant 博士與NIGHTSEA Charles Mazel博士提供
6. 用于結核分枝桿菌重組株的篩選
在結核分枝桿菌中成功構建了同源重組系統(tǒng)，利用該系統(tǒng)構建了rv1364c、pstP跨膜區(qū)、pstP胞外區(qū)三個突變株，得到雙交換突變株的效率為25％-62.5％，從雙交換突變株得到無痕缺失突變株的效率為100％。通過gfp作為熒光標記基因，利用NightSea BlueStar藍光手電筒和濾光眼鏡,可以對平板上的基因缺失株菌落直接進行快速判定。
采用nightsea BLS2 BlueStar 藍色手電筒和濾光鏡對平板上的結核分枝桿菌綠色熒光蛋白菌落進行快速鑒定。該方法可以從幾百個菌落中瞬間鎖定陽性克隆。平板上的菌落照片拍攝用單反相機加載購自nightsea公司的濾光片實現(xiàn)。
圖片源自[結核分枝桿菌同源重組基因敲除系統(tǒng)的構建和應用]
熒光是細胞生物學、神經(jīng)生物學等領域中廣泛使用的工具。美國nightsea致力于研究和開發(fā)熒光觀測裝備，其產(chǎn)品系列有DFP-1雙熒光蛋白手電筒系列，BLS2單熒光蛋白手電筒系列，SFA-RB體視顯微鏡熒光適配器等。
DFP-1 Dual Fluorescent Protein Flashlight 雙熒光蛋白手電筒系列
DFP-1雙熒光蛋白手電筒，有兩個可轉換的高強度LED，可激發(fā)高強度光源。目前，綠色熒光（GFP、EGFP、fluorescein等）和紅色熒光（DsREed，TdTomato等）是常用的類型，nightsea常規(guī)的DFP-1雙熒光蛋白手電筒是激發(fā)藍光和綠光，再分別使用黃色濾光鏡和紅色濾光鏡，就可以分別檢測綠色熒光蛋白（GFP、EGFP、fluorescein等）和紅色熒光蛋白（DsREed，TdTomato等）了。
Good News
標準的DFP-1雙熒光蛋白觀測鏡，只有RB-Royal Blue和GR-Green兩種熒光激發(fā)裝置，也就是說標準的DFP-1只能用于觀測綠色熒光蛋白和紅色熒光蛋白。
現(xiàn)在，您可以選擇以下任何兩種熒光裝置，組裝成自己專屬的雙熒光蛋白手電筒。
熒光裝置型號 | Excitation 激發(fā)光 | Emission 發(fā)射光 | 可觀測熒光 |
RB-Royal Blue | 440-460nm | 500nm LP | GFP, eGFP, fluorescein.... |
CY-Cyan | 490-515nm | 550nm LP | YFP, Venus, Lucifer Yellow... |
GR-Green | 510-540nm | 600nm LP | DsRed, dTomato... |
VI-Violet | 400-415nm | 460nm LP | CFP, BFP... |
UV-Ultra Violet | 360-380nm | 415nm LP | DAPI |
注：
1.RB-Royal Blue用于觀測綠色熒光蛋白
GFP：綠色熒光；EGFP：增強型綠色熒光；fluorescein熒光素
2.CY-Cyan用于觀測黃色熒光蛋白
YFP：黃色熒光蛋白；Venus：金色；Lucifer Yellow：熒光黃
3.GR-Green用于觀測紅色熒光蛋白
DsRed：紅色熒光蛋白；dTomato：番茄紅
4.VI-Violet用于觀測紫色熒光蛋白
CFP：青色熒光蛋白；BFP：堿性胎兒蛋白
5.UV-Ultra Violet用于觀測紫外光
DAPI：藍色熒光
nightsea 2018年 產(chǎn)品列表：
貨號 | 品名 | 規(guī)格 | 價格 | 品牌 |
SFA-TENT | Eclipse MicroTent | each | 5525 | nightsea |
SFA-EYE-S | Tru-Block Eye Shields - Standard | set | 306 | nightsea |
SFA-EYE-C | Tru-Block Eye Shields - Compact | set | 306 | nightsea |
SFA-BLH-RB | Light Head-Royal Blue | each | 4250 | nightsea |
SFA-BLH-CY | Light Head-Cyan | each | 4250 | nightsea |
SFA-BLH-GR | Light Head-Green | each | 4250 | nightsea |
SFA-BLH-VI | Light Head-Violet | each | 4250 | nightsea |
SFA-BLH-UV | Light Head-Ultraviolet | each | 6545 | nightsea |
SFA-BF-RB | NIGHTSEA Barrier Filter-Royal Blue | each | 3536 | nightsea |
SFA-BF-GO | NIGHTSEA Barrier Filter-Green Only | each | 3536 | nightsea |
SFA-BF-CY | NIGHTSEA Barrier Filter-Cyan | each | 3536 | nightsea |
SFA-BF-GR | NIGHTSEA Barrier Filter-Green | each | 3536 | nightsea |
SFA-BF-VI | NIGHTSEA Barrier Filter-Violet | each | 3536 | nightsea |
SFA-BF-UV | NIGHTSEA Barrier Filter-Ultraviolet | each | 3536 | nightsea |
SFA-SH-RB | NIGHTSEA Shield-Royal Blue | each | 1785 | nightsea |
SFA-SH-GO | NIGHTSEA Shield-Green Only | each | 1785 | nightsea |
SFA-SH-CY | NIGHTSEA Shield-Cyan | each | 1785 | nightsea |
SFA-SH-GR | NIGHTSEA Shield-Green | each | 1785 | nightsea |
SFA-SH-VI | NIGHTSEA Shield-Violet | each | 1785 | nightsea |
SFA-SH-UV | NIGHTSEA Shield-Ultraviolet | each | 1785 | nightsea |
SFA-AD | NIGHTSEA Adapter | each | 2856 | nightsea |
SFAZ-AD | Leica EZ4 Adapter only | each | 2856 | nightsea |
SFA-XL-AD | NIGHTSEA Oversize Adapter | each | 2856 | nightsea |
SFA-DIM-N | NIGHTSEA DIM Base | each | 11305 | nightsea |
SFA-PULSE-N | NIGHTSEA PULSE Base | each | 11305 | nightsea |
SFA-BASE | NIGHTSEA Standard Base | each | 7055 | nightsea |
SFA-LHH-L | Light Head Hanger, long working distance | each | 1615 | nightsea |
SFA-LHH-S | Light Head Hanger, short working distance | each | 1615 | nightsea |
SFA-LHC | Single Light Head Cable, V1 | each | 510 | nightsea |
SFA-LHC-BNC | Single Light Head Cable, BNC | each | 595 | nightsea |
SFA-DLHC | Dual Light Head Cable, V1 | each | 1105 | nightsea |
SFA-DLHC-BNC | Dual Light Head Cable, BNC | each | 1105 | nightsea |
SFA-HK-L | Single hanger kit, long working distance, V1: SFA-LHC + SFA-LHH-L | kit | 2125 | nightsea |
SFA-HK-S | Single hanger kit, short working distance, V1: SFA-LHC + SFA-LHH-S | kit | 2125 | nightsea |
SFA-HK-L-BNC | Single hanger kit, long working distance, BNC: SFA-LHC-BNC + SFA-LHH-L | kit | 2125 | nightsea |
SFA-HK-S-BNC | Single hanger kit, short working distance, BNC: SFA-LHC-BNC + SFA-LHH-S | kit | 2125 | nightsea |
SFA-DHK-L | Dual hanger kit, long working distance, V1: SFA-DLHC + 2x SFA-LHH-L | kit | 4165 | nightsea |
SFA-DHK-S | Dual hanger kit, short working distance, V1: SFA-DLHC + 2x SFA-LHH-S | kit | 4165 | nightsea |
SFA-DHK-L-BNC | Dual hanger kit, long working distance, BNC: SFA-DLHC-BNC + 2x SFA-LHH-L | kit | 4165 | nightsea |
SFA-DHK-S-BNC | Dual hanger kit, short working distance, BNC: SFA-DLHC-BNC + 2x SFA-LHH-S | kit | 4165 | nightsea |
SFA-SWK-S-BNC | Switch Box Kit, short working distance: BNC switch box, 3 BNC cables, one with female/female gooseneck adapter, 2 LH Hangers | kit | 5950 | nightsea |
SFA-SWK-L-BNC | Switch Box Kit, long working distance: BNC switch box, 3 BNC cables, one with female/female gooseneck adapter, 2 LH Hangers | kit | 5950 | nightsea |
DFP-1 | DFP Flashlight with RB and GR LEDs, matching filter glasses, carrying case | each | 12835 | nightsea |
DFP-CG | DFP Flashlight with CY and GR LEDs, matching filter glasses, carrying case | each | 13855 | nightsea |
DFP-RC | DFP Flashlight with RB and CY LEDs, matching filter glasses, carrying case | each | 13855 | nightsea |
DFP-VC | DFP Flashlight with VI and CY LEDs, matching filter glasses, carrying case | each | 13855 | nightsea |
DFP-VG | DFP Flashlight with VI and GR LEDs, matching filter glasses, carrying case | each | 13855 | nightsea |
DFP-VR | DFP Flashlight with VI and RB LEDs, matching filter glasses, carrying case | each | 13855 | nightsea |
DFP-UC | DFP Flashlight with UV and CY LEDs, matching filter glasses, carrying case | each | 16065 | nightsea |
DFP-UG | DFP Flashlight with UV and GR LEDs, matching filter glasses, carrying case | each | 16065 | nightsea |
DFP-UR | DFP Flashlight with UV and RB LEDs, matching filter glasses, carrying case | each | 16065 | nightsea |
DFP-UV | DFP Flashlight with UV and VI LEDs, matching filter glasses, carrying case | each | 16065 | nightsea |
BLS-1 | BlueStar light plus style VG1 filter glasses | each | 3485 | nightsea |
BLS-2 | BlueStar light plus style VG2 filter glasses | each | 3655 | nightsea |
BLS-3 | BlueStar light plus style VG3 filter glasses | each | 3655 | nightsea |
VG-1 | Yellow filter glasses, style 1 | each | 578 | nightsea |
VG-2 | Yellow filter glasses, style 2 | each | 935 | nightsea |
VG-3 | Yellow filter glasses, style 3 | each | 935 | nightsea |
RG-2 | Red filter glasses, style 2 | each | 935 | nightsea |
FG-UV | Filter glasses, Ultraviolet | each | 935 | nightsea |
FG-VI | Filter glasses, Violet | each | 935 | nightsea |
FG-GO | Filter glasses, Green Only | each | 935 | nightsea |
FG-CY | Filter glasses, Cyan | each | 935 | nightsea |
SFA-PULSE-R | PULSE Option - Retrofit | each | 4675 | nightsea |
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